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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP614056Yeast1mgQuote
EP614056E. coli1mgQuote
BP614056Baculovirus200ugQuote
MP614056Mammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia cenocepacia (strain AU 1054)
UniProt IDQ1BTS3
Gene NamemiaB; Locus:Bcen_2081
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRA SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTDIADFATLIEYVADIPGIERIRYTT SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP DLSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPREVK LKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS HPRLIGQMIDVKINHAYPHSLRGELVLVSDDASTATH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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