Request QuoteCatalog Number: xP641213NFDSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP641213NFDYeast1mgQuote
EP641213NFDE. coli1mgQuote
BP641213NFDBaculovirus200ugQuote
MP641213NFDMammalian Cell200ugQuote

Protein Information

SpeciesNovosphingobium aromaticivorans (strain DSM 12444)
UniProt IDQ2G9P6
Gene NamemiaB; Locus:Saro_0982
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-443
Expression Tag6xHis
Purity>90%
AA SequenceMSSSAPSSASPKTFRVKSFGCQMNVYDGERMAELLAAQGMSAAGDGDDADLVVLNTCHIR EKATEKVYSDIGRLRRADGSAPLIAVAGCVAQAEGEEIMARAPSVKVVVGPQSYHRLPEM VADAAAGKRSTETDMPAEAKFAALPKRRKSAPTAFLTVQEGCDKFCTYCVVPYTRGAEIS RPFSDLVEEAKLLVAGGAREITLLGQNVNAWAGEDDKGRPIGLDGLARALAAEPDLKRIR YTTSHPNDMTDGLIAAHGELEKLMPFLHLPVQAGNDRVLKAMNRSHTADSYMALLERIRA ARPDIALSGDFIVGFPGETDAEFEDTLRLVDAVGYAQAFSFKYSARPGTPAATMENHVPV AVMDERLQRLQAALNRDQLAFNKASVGKTCEVLVERRGKHPGQWLGKSPWLQSVHFSGEA EIGDMVTVELIEAGPNSISGRLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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