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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP612391Yeast1mgQuote
EP612391E. coli1mgQuote
BP612391Baculovirus200ugQuote
MP612391Mammalian Cell200ugQuote

Protein Information

SpeciesShewanella sp. (strain MR-4)
UniProt IDQ0HLI3
Gene NamemiaB; Locus:Shewmr4_1004
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVF HQLGRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAHCVDIIFGPQTLHRLPDMIEQVRR GEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDI QISSDFIIGFPGETKEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQ RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGLPKHIG SFVDVEIVDVYTNSLRGKFIRGEDEMDLRRNLRPSDILAKHKQDDDLGVTQFKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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