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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP607189Yeast1mgQuote
EP607189E. coli1mgQuote
BP607189Baculovirus200ugQuote
MP607189Mammalian Cell200ugQuote

Protein Information

SpeciesAlcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573)
UniProt IDQ0VN66
Gene NamemiaB; Locus:ABO_1934
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMARKLFIQTHGCQMNEYDSTRMVDLLESSHGLEPTDNPEEADVLLLNTCSIREKAQEKVF HQLGRWKKLKDAKPDMIIGVGGCVASQEGDAIRDRAPYVDVVFGPQTLHRLPGLITQAAS TRELAIDVTFPEIEKFDNLPEPSVDGPSAFVSIMEGCSKYCTFCVVPYTRGEEVSRPVQP VLKEIQHLADMGVREVNLLGQNVNAYQGVGADGDTLDLADLIRLIRDIDGIDRIRYTTSH PVEFSEALIQVYEDVPELVSHLHLPVQSGSDRILAMMKRNHMVLEYKSKLRKLKRIRPDI SFSSDFIIGFPGETDRDFEDTMNLIHDIGFDMSFSFIYSARPGTPAADLPDDVDMEVKKQ RLAILQQRINQNVQDISRKMVGSTQRILVDGFSKKDPGQLKGRTENNRVVNFQCDDTDLI GKFVDVTIAKAYSNSLLGTDPRNPS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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