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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP613067Yeast1mgQuote
EP613067E. coli1mgQuote
BP613067Baculovirus200ugQuote
MP613067Mammalian Cell200ugQuote

Protein Information

SpeciesClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
UniProt IDQ0TRE5
Gene NamemiaB; Locus:CPF_1349
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMTLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVREN AENKVFGNLGQLKQLKKKNPNLVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFP EYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVTIMYGCNNFCTYCIVPYVRGR ERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERV RFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIK SEIPDVTLTTDIIIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIP DDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAG DEKLVGELVNLKIVRAQPFSLIGEIVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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