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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP622964Yeast1mgQuote
EP622964E. coli1mgQuote
BP622964Baculovirus200ugQuote
MP622964Mammalian Cell200ugQuote

Protein Information

SpeciesFrancisella tularensis subsp. tularensis (strain FSC 198)
UniProt IDQ14IK5
Gene NamemiaB; Locus:FTF0618c
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEK VFHELGRWKGLKKTNEDLIIGVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQK QKTQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCDKYCSYCVVPYTRGPEVNRPF EDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTT SHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRP DITISSDFIVGFPGETEEDFQKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVK KDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDNILSGRTENNRVVNFKGDKSL IGQFAMVKITESLPNSLRGELI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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