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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP312281BNAYeast1mgQuote
EP312281BNAE. coli1mgQuote
BP312281BNABaculovirus200ugQuote
MP312281BNAMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Cinara cedri (strain Cc)
UniProt IDQ057G5
Gene NamemiaB; Locus:BCc_272
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-435
Expression Tag6xHis
Purity>90%
AA SequenceMNEHDSSIIENILKKTNLYIITKKPEISDILILNTCSIREKAQEKLFHQLGRWKKLKQKN SKILIAVGGCVAVQEGKKIYKRAKFIDIIFGPQTLHKLPKLLIESNKKKSLIINIKKKSL KKFNYTINKNTNIKKKFSSFVTIMEGCNKYCSFCIVPYTRGKEVSRNNKKIISEIIELSK KGVREITLLGQNVNAYKFSDTFNKKNYSFSDLLYSISEIPRIDRIRFITSHPVEFNNNII EAYKKIPKLTNFLHLPVQSGSNKILKLMKRGYTIEKYENIVNKIKKIRPKINISSDFIIG FPGETKEDFQKTIYFISKINFDTSYSFIYSKRPRTRASKLEDNVTMEEKKKRLYKVQQKI NQQAFQWKRRSTEQIVLVEGISKNNIQELYGRTENNRTVFFEGNPKFIGNFIKLKIISIK YNTFLKGKIISNNYF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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