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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP642629Yeast1mgQuote
EP642629E. coli1mgQuote
BP642629Baculovirus200ugQuote
MP642629Mammalian Cell200ugQuote

Protein Information

SpeciesSyntrophus aciditrophicus (strain SB)
UniProt IDQ2LT94
Gene NamemiaB; Locus:SYNAS_14210; ORFs:SYN_02149
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-461
Expression Tag6xHis
Purity>90%
AA SequenceMMKSSNKSSGSLIRLDKKHIYIQTLGCQMNVHDSEQIAALMEEKGYICTEDANEADLIIL NTCSIREKAAQKAKSQLGRYRNLKRKKRNLLIGVGGCLAQQLGDELLTKVPDIDFIFGTH NIHQLPDFISRIEKSRKKIVETTLHPSTPSIGVLALPCNGQVSSFVTIMQGCNNFCSYCI VPYVRGREESRPPEDIIHEIRMLADHGVKEVTLLGQNVNSYARKTSGEMGFAELLREIEK IKGIERMRFTTSHPKDLSEFLITAFSDLSKLCHHIHLPFQSGSDRILALMNRGYTKSDYL AKVERLRTVCPDISITADVIVGFPGESDEDFKETIDMMNQIRFDNLFSFKYSEREGTAAV KMDGKVSEPLKLERLQILQALQEQHTLEKNKAMEGKQEDVLVEGFSKNCRKDLTGRTSTN KIVNFSGCVDLIGDMVSVLIKEAYLHSLRGEMLCEEVVHAN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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