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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP621713Yeast1mgQuote
EP621713E. coli1mgQuote
BP621713Baculovirus200ugQuote
MP621713Mammalian Cell200ugQuote

Protein Information

SpeciesRhodopseudomonas palustris (strain BisB5)
UniProt IDQ13EK7
Gene NamemiaB; Locus:RPD_0242
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMAPPRKLHIKSYGCQMNVYDAQRMVDVLAPEGFVETATVDDADLVILNTCHIREKASEKV YSELGRLRLARDEAASSGRRMQIAVAGCVAQAEGEEIVRRAPVVDVVVGPQSYHHLPQLL ARADQAGRALETEFPVEDKFGFLPQPRPETIRARGISAFVTVQEGCDKFCTFCVVPYTRG AEVSRPVSAIIDDVKRLADNGVREITLIGQNVNAYHGEGPDSRAWTLGRLLRRLAAVPGI VRLRYSTSHPNDVDDELIEAHRDLDALMPFVHLPVQSGSDPILAAMNRKHTAADYRRVID RFRAVRPQIAFSSDFIVGFPGETEADFAATLALVTQIGYAGAYSFKYSPRPGTPAADMQE MVPAAVMDERLERLQQLIDSQQSAFNKAAIGQTVDVLFERAGRKPGQIVGRTAYLQPAHV FPGPMFLGPGMAPDSLVGQILPVRVDSLERYSLLGELAAKPPQHARPLAATGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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