Request QuoteCatalog Number: xP621391YAFSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP621391YAFYeast1mgQuote
EP621391YAFE. coli1mgQuote
BP621391YAFBaculovirus200ugQuote
MP621391YAFMammalian Cell200ugQuote

Protein Information

SpeciesYersinia pestis bv. Antiqua (strain Antiqua)
UniProt IDQ1C531
Gene NamemiaB; Locus:YPA_2477
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVF SLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQG TNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD ILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSH PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDI QISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQ RLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIG KFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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