Request QuoteCatalog Number: xP600529SYTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP600529SYTYeast1mgQuote
EP600529SYTE. coli1mgQuote
BP600529SYTBaculovirus200ugQuote
MP600529SYTMammalian Cell200ugQuote

Protein Information

SpeciesShewanella frigidimarina (strain NCIMB 400)
UniProt IDQ087J4
Gene NamemiaB; Locus:Sfri_0713
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLDDYQGYTLTEDASEADVLLLNTCSIREKAQEKVF HQLGRWKSLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQIQR GEKAVIDISFPEIEKFDRLPEPRADGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD VILEVAQLAAQGVREVNLLGQNVNAYRGATHDDEICTFAELLRLVASIDGIDRLRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKARPDI QISSDFIIGFPGESKDDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVSDEEKKQ RLAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTENSRVVNFEAAHTHIG SFVDVKIVDVYTNSLRGEFVRGENEMDLRRSLRPAEILAKHKQDDALGVTHFKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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