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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP607673Yeast1mgQuote
EP607673E. coli1mgQuote
BP607673Baculovirus200ugQuote
MP607673Mammalian Cell200ugQuote

Protein Information

SpeciesCytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
UniProt IDQ11U26
Gene NamemiaB; Locus:CHU_1821
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-481
Expression Tag6xHis
Purity>90%
AA SequenceMKQVVQDLDVLPAAPTAAEKAACDLPRITEERNTGKQRKLYIESYGCQMNFSDSEIVMSI LTEEGFDTTSTPNNADVILLNTCSIRDNAEQKVRHRLVHFKGLKRNKPSMIVGVLGCMAE RLKAQLLEEEKIVDMVVGPDAYRDLPNLINEVDSGHKAVNVFLSREETYADINPLRLSGD GVSAFVSIMRGCDNMCSFCVVPYTRGRERSRDAHSILNEVRELVANGYKEVTLLGQNVDS YKWSAEGTDERVNFAQLLERVALVDPDLRVRFSTSHPKDITDEVLYTMKKYDNICNSIHL PAQSGNTRILELMNRTYTREWYMERITKIRQILGDDCGISSDTIAGFCSETEEEHQDTLS LMEFARFDFGYQFVYSERPGTPAAKKLADDVPEDVKQRRLSEIITVQRQLSLEHNQAQIG TIQKILIEGVSKKSEHDLKGRNSRNMMAIFPKKNYSKGQYVDVLITGCSGGTLFGEIVET V
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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