Request QuoteCatalog Number: xP350066BWZSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP350066BWZYeast1mgQuote
EP350066BWZE. coli1mgQuote
BP350066BWZBaculovirus200ugQuote
MP350066BWZMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
UniProt IDP57516
Gene NamemiaB; Locus:BU441
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-439
Expression Tag6xHis
Purity>90%
AA SequenceMKYIYIKTWGCQMNEYDSSMIITLLEKNNQYSLTKSAENADILILNTCSIREKAQEKVFH QLGRWKKIKNNNPKVIIAVGGCVATQEGKEIFKRANYVDIIFGTQTLHRLPKMIDEVEKK RKLSIDISFPKLEKFKYFLAPKKKGYTADISIMEGCNKYCSFCVVPYTRGNEISRPCDDV LFEISLLAKQGIKEINLLGQNVNAYQGPTFNGKVCYFSELIRLVAEIDGIERIRFTTSNP LEFTDDIIEVYKDTPKLVSFLHLPVQSGSNKILNLMKRSYTTEDYTSIIKKLTIARPDIQ ISSDFIVGFPGESEIDFEKTIEFIKNINFDMSFSFIYSARPGTPASNMNDDLDLKEKKRR LYILQERINIQTMLWSRKMFGSIQSVLVEGVSDKNIMDLYGRTENNRVVTFQGSSKMIGQ FVNVKIKKVHTHSLKGELF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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