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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP530655TPRYeast1mgQuote
EP530655TPRE. coli1mgQuote
BP530655TPRBaculovirus200ugQuote
MP530655TPRMammalian Cell200ugQuote

Protein Information

SpeciesTreponema pallidum (strain Nichols)
UniProt IDO83735
Gene NamemiaB; Locus:TP_0754
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMTYFFETYGCQMNVAESASVEQLLLARGWTKAVDAQTCDVLIINTCSVRITAETRVFGRL GLFSSLKKKRAFFIILMGCMAQRLHDKIQQQFPRIDYVVGTFAHARFESIFQEIEQKLTQ KDYRFEFISERYREHPVSGYRFFASSYSEGSFQSFIPIMNGCNNFCSFCIVPYVRGREIS RDLDAILQEVDVLSEKGVREITLLGQNVNSYRGRDREGNIVTFPQLLRHLVRRCEVKDQI KWIRFVSSHPKDLSDDLIATIAQESRLCRLVHLPVQHGANGVLKRMRRSYTREQYLSLVG KLKASVPNVALSTDILIGFPGETEEDFEQTLDLMREVEFDSAFMYHYNPREGTPAYDFPD RIPDATRIARLQRVIALQMSTTLKKMRARVGKTLPVLVESRSRNNPEELFGHTELGEMTV LEGKVDPTYIGRFVDVQVKEVRGRTLRAHLVQERAK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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