Request QuoteCatalog Number: xP456928DUFSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP456928DUFYeast1mgQuote
EP456928DUFE. coli1mgQuote
BP456928DUFBaculovirus200ugQuote
MP456928DUFMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain Alaska E43 / Type E3)
UniProt IDB2V276
Gene NamemiaB; Locus:CLH_1699
Protein Name
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMNFDIEKLEKIKVRDGQEFFFIQTFGCQMNEEDSEKLSGMLKSQGYEETENRDEASIVIF NTCCVRENAENKVFGNLGRLKNQKEKNPNLIIALCGCMMQQKGMADEILSRFPYVDIIFG THNAYKFPEYLHRVQVEGVQVKEIFDKETEIVEGVPIDRKSNVKAFVTIMYGCNNFCTYC VVPYVRGRERSRRPEDIENEIKELVSNGYKEITLLGQNVNSYGKGLEEEITFAQLLRRIN EIDGLERLRFMTSHPKDLTLDVVYAIRDCDKLCEQIHLPVQSGSNEILQKMNRHYNKEQY LELAKKIREEIPDVTFSTDIIVGFPGETEEDFEETINLVKEVRYDAAFTFIYSRRNHTPA DKMEDQIPDDVKHDRFNRLVAAVNEGIVVGNKAAEGKIYEVLVEGTSKNNENKLTGRTRN AKLVNFDGCKEMIGKLVKVKIIEAKSFSLVGEVVEQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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