Request QuoteCatalog Number: xP470017BQPSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP470017BQPYeast1mgQuote
EP470017BQPE. coli1mgQuote
BP470017BQPBaculovirus200ugQuote
MP470017BQPMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain G9842)
UniProt IDB7ITM4
Gene NamemiaB; Locus:BCG9842_B1428
Protein Name
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMNEQQRLASQQANSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTTLGYEPTFSTEDADVVLLNTCAIRENAENK VFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDVEYGLGDLMDELRKVDIARIRFTT SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIP DAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK KERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSL IGQLVKVKVTEAKTWSLNGELVEEPIEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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