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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP459686EUPYeast1mgQuote
EP459686EUPE. coli1mgQuote
BP459686EUPBaculovirus200ugQuote
MP459686EUPMammalian Cell200ugQuote

Protein Information

SpeciesPhenylobacterium zucineum (strain HLK1)
UniProt IDB4RC70
Gene NamemiaB; Locus:PHZ_c3454
Protein Name
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMSEPAPAKRLYIKTYGCQMNVYDSERMADVLAPLGYGVTDDPAAADLVVLNTCHIREKAT EKVYSELGQIKRLKEARRAEGQGMTIAVAGCVAQAEGEEIMRRQPAVDLVVGPQAYHQLP ELIARAHRARGERLAADFAPDEKFDALATERRPTGVTAFLTVQEGCDKFCTFCVVPYTRG GEWSRPAEAIEAEARALAAKGVREVTLLGQNVNAYDGANGQGAGLAGLVRRLAKIPGLDR IRYTTSHPRDMDDDLIAAHAEVPELMPYLHLPVQAGSDRILRAMNRAHTAESYLRVIEKV RVARPDIAISGDFIVGFPGEREADFEATLQLVREVGYASCFSFKYSRRPGTPAAALPGQV AEEVKEERLQRLQALLEQQQLAFNAAQAGRVLPVLFEKTGRHPGQLIGRSPYLQAVHAHA PDRLIGQIVPVKVESGGRNSLAGVLELETA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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