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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP478297DXNYeast1mgQuote
EP478297DXNE. coli1mgQuote
BP478297DXNBaculovirus200ugQuote
MP478297DXNMammalian Cell200ugQuote

Protein Information

SpeciesCoxiella burnetii (strain CbuK_Q154) (Coxiella burnetii (strain Q154) )
UniProt IDB6J853
Gene NamemiaB; Locus:CbuK_1268
Protein Name
Region Expressed1-439
Expression Tag6xHis
Purity>90%
AA SequenceMKKLYLKTHGCQMNEYDSAKMADVLKFSHGLELTEDPAVADVFLLNTCSVREKAQTKVFS ELGRWRPFKEKRPHVVIGVGGCVASQEGETILKQAPFVDIVFGPQTLHRLPDLLDSVIQK RKSVVDITFPEIEKFDRLPQPRAEGPSAFVSIMEGCSKYCTFCVVPYTRGEEISRPFDDV IAEVASLCEQGVREITLLGQNVNDYRGLMHDGQVADLALLIHYLAAMDNIERIRFTTSHP SAFSENLIDAYAEEPKLANHLHLPVQSGSDRILAAMKRNYTVLEYKSKIRKLRAVRPDIS LSSDFIIGFPGETDADFEATMNLIHDMGFDHSFSFIYSPRPGTPAAQLPDDVPMAVKKER LAILQNRINAKAAEISQSMVGTQQRILVTGPSKKYPDQLSGRTENNRVVNFNGDTPLIGQ MVTIKIKEARPYSLWGEIC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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