Request QuoteCatalog Number: xP540918FOFSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP540918FOFYeast1mgQuote
EP540918FOFE. coli1mgQuote
BP540918FOFBaculovirus200ugQuote
MP540918FOFMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
UniProt IDB1VXU5
Gene NamemiaB; Locus:SGR_1732
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-506
Expression Tag6xHis
Purity>90%
AA SequenceMSSGNRSEAVDVQKSYEVRTYGCQMNVHDSERLSGLLEGAGYVRAPEGSDGDADVVVFNT CAVRENADNKLYGNLGRLAPMKTKRPGMQIAVGGCLAQKDRDTIVKRAPWVDVVFGTHNI GKLPVLLERARVQEEAQIEIAESLEAFPSTLPTRRESAYAAWVSISVGCNNTCTFCIVPA LRGKEKDRRTGDILAEIEALVAEGVCEITLLGQNVNAYGSDIGDREAFSKLLRACGRIEG LERVRFTSPHPRDFTDDVIAAMAETPNVMPQLHMPMQSGSDTVLKAMRRSYRQERFLGII EKVRAAMPEAAISTDIIVGFPGETEEDFEQTMHAVREARFANAFTFQYSKRPGTPAADMD GQIPKEVVQERYMRLSALQEQISWDENKKQVGRTLDVMVAEGEGRKDGATQRLSGRAPDN RLVHFTQPEKAVRPGDVVTVEITYAAPHHLLAEGTPLAVRSTRAGDAWEKRTTGAAAKPA GVMLGLPGIGAPEPLPAAGAPACGIG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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