Request QuoteCatalog Number: xP464655RJISize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP464655RJIYeast1mgQuote
EP464655RJIE. coli1mgQuote
BP464655RJIBaculovirus200ugQuote
MP464655RJIMammalian Cell200ugQuote

Protein Information

SpeciesRalstonia pickettii (strain 12J)
UniProt IDB2UFP3
Gene NamemiaB; Locus:Rpic_0408
Protein Name
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMKKVFIKTYGCQMNEYDSDKMSDVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFS ELGRVKALKALKPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPDLIAARRRT GRSQVDVSFPEIEKFDHLPPARVDGASAYVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDV LAEVAGLAEQGVREVTLLGQNVNAYIGKMGDTSERADFALLLEYVAEIPGIERIRYTTSH PKEFSSRLIEAYATNPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSSIRKLRAIRPNI SIATDFIVGFPGETDADFAKTMDLIHEIGYDTSFSFIYSPRPGTPAANLHDDTPQAVKLE RLKHLQATIEENVARISQGMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPDLPQV RREQLIGQMLDVRIVHAFPHSLRGEVAEERMASAAH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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