Request QuoteCatalog Number: xP472471BQMSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP472471BQMYeast1mgQuote
EP472471BQME. coli1mgQuote
BP472471BQMBaculovirus200ugQuote
MP472471BQMMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain AH820)
UniProt IDB7JJ50
Gene NamemiaB; Locus:BCAH820_3784
Protein Name
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK VFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK KERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL IGQLVKVKVTDAKTWSLNGELVEEPIEVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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