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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP478868AWHYeast1mgQuote
EP478868AWHE. coli1mgQuote
BP478868AWHBaculovirus200ugQuote
MP478868AWHMammalian Cell200ugQuote

Protein Information

SpeciesAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270) )
UniProt IDB7J5B2
Gene NamemiaB; Locus:AFE_0596
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-451
Expression Tag6xHis
Purity>90%
AA SequenceMQNLYIKTYGCQMNEYDSERMADTLAVSHGLRLVDDPVLADVLLLNTCSIREKAEDKVFT QLGFWRPFKERRPEVVIGVGGCVASQEGERLRRRAPYVDLVFGPQTLHRLPDLLDACLAE RRPQVDIAFPMLEKFDHLPQRPGRDGATAFVTIQEGCDKFCTFCVVPHTRGREYSRSMPD ILREVRALVEQGVREITLLGQNVNAYRGATGLVGEGGLADLLERLARIPGLLRLRYTTSH PANLDDELIAAHGSIGILAPHLHLPVQSGSDRILRRMHRKHTVGQYLDKVDRLRAARPGI QISSDFIVGFPGETDADFAATMELIDAVRFDQSFSFKYSQRPNTPALKLKDSVPEAVKED RLAVLQGRINGLAQGYAQALVGTQQAVLITGPSRRDAQELTGKTACNRAVNLAGSMDWVG QMLDVEITAALPNSLRGRAALVAPTSQRLAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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