Request QuoteCatalog Number: xP462968MSVSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462968MSVYeast1mgQuote
EP462968MSVE. coli1mgQuote
BP462968MSVBaculovirus200ugQuote
MP462968MSVMammalian Cell200ugQuote

Protein Information

SpeciesMethylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4) )
UniProt IDB3E0M0
Gene NamemiaB; Locus:Minf_0719
Protein Name
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMPSVFIKTFGCQMNVRDSEQVLQDFIERGYQIASSEKWADIILINTCSVRAMAEEKAIDK LASLKTAKKKNPNLVLGIIGCMAQNRGREIAEKYRFVDLVLGTQKFHKVAEIADNLLKNP DRSSSYVDLSKEEAAHNAINKHLSTKAQPIAYVSIMQGCSMHCSFCIVPTTRGEERSRPI DEIFEEVKRLAETSVKEIVLLGQIVNRYGAKEFPWVKGKSPFVQLLEKLSTIEEIKRIRF TSPHPLGFKEDLIAALRDIPQLCEHVHLPVQSGSDKILKAMRRGYSRSKFLSLVDKLRKA IPQLALSTDIIVGYPGETEEDFQQTCSLLNEVRFDNAFIFRYSAREGTTAASLGDQLSEE VKFERNYRLLEIQNKITMEKAQKWVGQVVEILVEGESKKNASKFQGRTRTNHLVIIPKNE RWRGEFLPVRIVETTGHTFYGTPLISGIDEALQFDLQEEINPAPIVS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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