Request QuoteCatalog Number: xP459035OEVSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP459035OEVYeast1mgQuote
EP459035OEVE. coli1mgQuote
BP459035OEVBaculovirus200ugQuote
MP459035OEVMammalian Cell200ugQuote

Protein Information

SpeciesOrientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
UniProt IDB3CV38
Gene NamemiaB; Locus:OTT_1777
Protein Name
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYS ELGRIKKLQQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLE KLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQEGCDKFCTFCVVPYTRGAEF SRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHSRERYLEIIQQLR ELRPDIVISSDIIVGFPGEDDEDFEATLSLAKEARFGQCYSFKYSQRPGTPAAVKQQISE EVKQHRLSILQAQLMQQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK DNNLYGKIVNVKILTASASSLFGEVYADS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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