Request QuoteCatalog Number: xP479549AWISize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP479549AWIYeast1mgQuote
EP479549AWIE. coli1mgQuote
BP479549AWIBaculovirus200ugQuote
MP479549AWIMammalian Cell200ugQuote

Protein Information

SpeciesAcidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993) )
UniProt IDB5ENG4
Gene NamemiaB; Locus:Lferr_0748
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-451
Expression Tag6xHis
Purity>90%
AA SequenceMQNLYIKTYGCQMNEYDSERMADTLAVSHGLRLVDDPVLADVLLLNTCSIREKAEDKVFT QLGFWRPFKERRPEVVIGVGGCVASQEGERLRRRAPYVDLVFGPQTLHRLPDLLDACLAE RRPQVDIAFPMLEKFDHLPQRPGRDGATAFVTIQEGCDKFCTFCVVPHTRGREYSRSMPD ILREVRALVEQGVREITLLGQNVNAYRGATGLVGEGGLADLLERLARIPGLLRLRYTTSH PANLDDELIAAHGSIGILAPHLHLPVQSGSDRILRRMHRKHTVGQYLDKVDRLRAARPGI QISSDFIVGFPGETDADFAATMELIDAVRFDQSFSFKYSQRPNTPALKLKDSVPEAVKED RLAVLQGRINGLAQGYAQALVGTQQAVLITGPSRRDAQELTGKTACNRAVNLAGSMDWVG QMLDVEITAALPNSLRGRAALVAPTSQRLAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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