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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP464339EXZYeast1mgQuote
EP464339EXZE. coli1mgQuote
BP464339EXZBaculovirus200ugQuote
MP464339EXZMammalian Cell200ugQuote

Protein Information

SpeciesPorphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257)
UniProt IDB2RKG6
Gene NamemiaB; Locus:PGN_1342
Protein Name
Region Expressed1-465
Expression Tag6xHis
Purity>90%
AA SequenceMIEQIGADSKSAENKQERKLYIETYGCQMNVADSEVVASVMQMDGYSLTDNVDEADTILV NTCSVRDNAEQKVLNRLAYYHSLRKKRRASSRLVIGVLGCMAERVKEELIREHHVDVVAG PDSYLDLPNLVGAAEQGEKAINVELSMQETYKDVMPLKMGGVHINGFVSIMRGCNNFCSY CIVPYTRGRERSREIESILNEVRDLKAKNFREVTLLGQNVNSYRYEQNGRIIRFPDLLAA VAEAVPDMRIRFTSPHPKDMDDEAIAVMARYRNICNHIHLPAQSGSDKMLRVMKRGYTRR WYLDRVAAIRRAIPDCAISSDLFCGFHSETEEDFEATLSLMEEVGYDSAFMFKYSERPGT YAARHLVDDVLEEVKLARLDRMIALQNRLSEESNKRDISKTFEVLIEGFSKRSREQLFGR TQQNKVVIFDKNGHRVGQYIYVRIKDASSATLFGEVVEAPTSEKG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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