Request QuoteCatalog Number: xP462176BSPSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462176BSPYeast1mgQuote
EP462176BSPE. coli1mgQuote
BP462176BSPBaculovirus200ugQuote
MP462176BSPMammalian Cell200ugQuote

Protein Information

SpeciesBeijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712)
UniProt IDB2IIK5
Gene NamemiaB; Locus:Bind_1055
Protein Name
Region Expressed1-510
Expression Tag6xHis
Purity>90%
AA SequenceMSGFNDSPVPESAEKADGLLSQERGTQDPLVNTRKLFVKSYGCQMNVYDAERMADLLAPE GYAETSAPEDADLVILNTCHIREHAAEKVFSELGKLRLLKAEQQAAGRPVKIVVAGCVAQ AEGEEILRRQKAVDLVVGPQSYHRLPDLLRRVAHTPGLVDTEFPAEDKFDHLVAPQPEKI AERGVGAFVTVQEGCDKFCSFCVVPYTRGAETSRPVEAILAEVETLIASGVREVTLIGQN VNAYHGFDAMTGAPASLASLMARVAAMPGLLRIRYTTSHPNDMGEDLIAAHRDIPALMPF LHLPVQSGSDKILAAMNRRHKAGDYLELIASIRAARPDIALSSDFIVGFPGETEADFEAT LALIEQVGFASAFSFKYSQRPGTPGADRPDQIDEDVKAQRLARLQALLEEQRQAFNKAMI GRVLPVLFEKPGRHPGQIAGKTPYLQALYAEGDKALIGTVQPVEILEAGPNSFHGRLLAR ETGQESAQGQESAQGMERMEQNARAWEVPV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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