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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462106AWOYeast1mgQuote
EP462106AWOE. coli1mgQuote
BP462106AWOBaculovirus200ugQuote
MP462106AWOMammalian Cell200ugQuote

Protein Information

SpeciesAcinetobacter baumannii (strain ACICU)
UniProt IDB2HZ55
Gene NamemiaB; Locus:ACICU_03225
Protein Name
Region Expressed1-483
Expression Tag6xHis
Purity>90%
AA SequenceMTVQTFIPNGAKAASENTVTQPTHTTDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGY VLTNNPNEADILLMNTCSIREKAQEKVFSELGRWRKLKEQNPDLVIGVGGCVASQEGDNI QKRAPYVDMIFGPQTLHRLPQMLDQHHAQVEKPKKEKIKLVDISFPDIEKFDFLPEPRVE GFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG YRGETFEGGICTFPELLRLVAEIPGIGRLRYTTSHPLEFSDELIQCYEDLPQMVSHLHLP VQSGSNDVLKAMKRNHTIDVYIDKIAKLRKIRPDMHLSSDFIIGFPGETDENFAETLQFI KDLDFDHSYSFVYSKRPGTPASDLPDTTPEHVKKERLAQVQQVIKQSSIEKTDAMLGKIE RVLIEKVSDQDPNILVGTADNTRLVTFVGDASWIGRFAEIEITEIKTLNLVYGELLNLEP DVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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