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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP540651TNMYeast1mgQuote
EP540651TNME. coli1mgQuote
BP540651TNMBaculovirus200ugQuote
MP540651TNMMammalian Cell200ugQuote

Protein Information

SpeciesThermotoga sp. (strain RQ2)
UniProt IDB1L8F3
Gene NamemiaB; Locus:TRQ2_0276
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-443
Expression Tag6xHis
Purity>90%
AA SequenceMRFYIKTFGCQMNENDSETMAGLLMKEGFTPASAPEEADVVIINTCAVRRKSEEKAYSEL GQMLKIKRKRKLVVGVAGCVAEKEREKLLERGADFVLGTRAVLKVTEAVKRALQGEKVAL FEDHLDEYTHELPRIRSSKHHAWVTIIFGCDRFCTYCIVPYTRGREKSRPMEDILEEVRE LAKQGYREVTFLGQNVDAYGKDLKDGSSLAKLLEEASKIEGIERIWFLTSYPTDFSDELI EVIARNPKVAKSVHLPVQSGSNRILKLMNRSYTKEEYLALLERIRSKVPDVAISSDIIVG FPTETEEDFMETIDLVEKAQFERLNLAIYSPRKGTVAWKHYKDEVPYEEKVRRMQFLMNL QKRINRKLNERYKGKTVRVIVEAQAKNGLFYGRDIRNKIIAFEGEEWMIGRFADVKIEKI TAGPLYGKVVWIEETPSPVSTDK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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