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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462253BXTYeast1mgQuote
EP462253BXTE. coli1mgQuote
BP462253BXTBaculovirus200ugQuote
MP462253BXTMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia phymatum (strain DSM 17167 / STM815)
UniProt IDB2JD88
Gene NamemiaB; Locus:Bphy_0412
Protein Name
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVF SDLGRVRELKEANPNLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDQRRA SGRPQVDITFPEIEKFDHLPPARIDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDD VLTEIAGLADQGVREVTLLGQNVNAYRGAMTLGATEIADFATLIEYVADIPGIERIRYTT SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP DLSLSTDMIVGFPGETEEDFDKMMQLVHDMSYDTSFSFIYSPRPGTPAANLQDDTPREVK LRRLQHLQATIEENVQRISQAMVGKVERILVERPARKDPNELAGRTENNRVVNFPAPVET HARLIGQMIDVKIVHAYPHSLRGELVMVHDTAPATH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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