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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP453509RKLYeast1mgQuote
EP453509RKLE. coli1mgQuote
BP453509RKLBaculovirus200ugQuote
MP453509RKLMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium etli (strain CIAT 652)
UniProt IDB3PZB6
Gene NamemiaB; Locus:RHECIAT_CH0000416
Protein Name
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMTQDSALLQAPEPMLRDGSNSRKVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEAD LVLLNTCHIREKAAEKVYSALGRLREMKKKKAADGREMMIGVTGCVAQAEGEEILRRAPA VDVVIGPQTYHRLPEALRRAQQGQRVVDTEYAIEDKFEHLPIAESRKIRARGVTAFLTVQ EGCDKFCTFCVVPYTRGSEVSRPVSQIVEEAEKLVEAGVREITLLGQNVNAWHGAGPRGE AWSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLIEAHRDLRALMPYLHLPVQSGSDRIL KAMNRRHTAAEYLSLIERIRTVRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAF SFKYSTRPGTPGAELKDQVPEEIKAERLERLQALLLKQQQEFAESCIGKEIDLLLEKPGR MPGQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGTNSLFAERAEAAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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