Request QuoteCatalog Number: xP462459BWRSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462459BWRYeast1mgQuote
EP462459BWRE. coli1mgQuote
BP462459BWRBaculovirus200ugQuote
MP462459BWRMammalian Cell200ugQuote

Protein Information

SpeciesBrucella abortus (strain S19)
UniProt IDB2S9E5
Gene NamemiaB; Locus:BAbS19_I20150
Protein Name
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDA DLVLLNTCHIREKASEKLYSALGRLRKMRDARAADGKELTIGVAGCVAQAEGQEILRRAP NVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHLPAPRREETRKRGVSAFLTV QEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG REWGLGELLFRLARIPGIARLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRI LKAMNRRHKADEYLRLIERIRNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQA YSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQYAFQDSMIGRKMDVLLEKPG REAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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