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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP455410DQYYeast1mgQuote
EP455410DQYE. coli1mgQuote
BP455410DQYBaculovirus200ugQuote
MP455410DQYMammalian Cell200ugQuote

Protein Information

SpeciesCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
UniProt IDB3PER6
Gene NamemiaB; Locus:CJA_1640
Protein Name
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMSATQTPTKKLYIKTHGCQMNEYDSNRMRDLLGESHNMVATENPEEADVILINTCSIREK AQEKLFHELGRWKNLKKQNPELIIGVGGCVASQEGAAIAERAPYVDLIFGPQTLHRLPEM METKKSNGVVVVDISFPEIEKFDKLPQPDADGVSAFVSIMEGCSKYCTFCVVPYTRGEEV SRPVADVMAEVIHLAKQGVREVNLLGQNVNAYRGAAADGTIVDLAELITYIAAVDGIDRI RFTTSHPVEFTDALIEVYNQVPELVSHLHLPVQSGSDRILMAMKRGHTVLEYKSKLRRIK KNRPNISFSSDFIIGFPGETDADFEATMKLIHDMEFDTSFSFIYSPRPGTPAADLPDDTP EEVKKQRLAILQDRITQQAMAISRRMVGNTERILVSGYSKKDPGQLSGRTENNRVVNFRC DNPALIGKFADVLIEEALPNSLRGSLIASELDQDWH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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