Request QuoteCatalog Number: xP454184DZSSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP454184DZSYeast1mgQuote
EP454184DZSE. coli1mgQuote
BP454184DZSBaculovirus200ugQuote
MP454184DZSMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424) )
UniProt IDB2AHA4
Gene NamemiaB; Locus:RALTA_A0485
Protein Name
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEATDNVEDADVILFNTCSVREKAQEKVFS ELGRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRT GQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV LAEVAGLAEQGVREVTLLGQNVNAYRGKMGDTSEIADFALLIEYVAEIPGIERIRYTTSH PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRILMAMKRGYSVLEYKSIIRRLRALRPDM SMSSDFIVGFPGETDADFDKLMAMIEEIGYDTSFSFIFSPRPGTPAANLHDDTPREVKLQ RLQRLQATIEENVQRISQGMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQA GRDRLVGQLVDVSITQAFPHSLRGEIVVRQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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