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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP454163NFOYeast1mgQuote
EP454163NFOE. coli1mgQuote
BP454163NFOBaculovirus200ugQuote
MP454163NFOMammalian Cell200ugQuote

Protein Information

SpeciesNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
UniProt IDB2A3X6
Gene NamemiaB; Locus:Nther_1495
Protein Name
Region Expressed1-451
Expression Tag6xHis
Purity>90%
AA SequenceMTLYQLGNGKKFYTLTFGCQMNEHDSEVLAGMLDQMGFEKAASEEEADLLIINTCAVREK AEQKVLGKIGTLRYLKENKPDMKIAIGGCMVQQEHVANKIYRDFTHVDIIFGTHNINRFP QLLEHVMQKGKRVKEISQDDSQVFENLPHKREDSIKAWVVISYGCDNYCKYCIVPYVRGQ QRSRDPEHIKYEVEKLAKEGLKEITLLGQNVNSYGKDLDQNISFTNLLEELSKIEGIERI RFMTSHPKDFDKELITTLKESNKICEHFHLPVQAGSNKILKKMGRGYTREHYVDIVNDIR AELPNASITTDIIVGYPGEEEEDFQETLDLVQNVKFDSAFTFVYSKRSGTPAAEMAEQVD EQTKKGRIQKLISVQQEISEQRNKDLENTVQRILVEGVSKNNEDMLSGRTRTDKLVHFPG DKELIGELVDVKITRGHSWNLYGEIFEDSLT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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