Request QuoteCatalog Number: xP458001OENSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP458001OENYeast1mgQuote
EP458001OENE. coli1mgQuote
BP458001OENBaculovirus200ugQuote
MP458001OENMammalian Cell200ugQuote

Protein Information

SpeciesOpitutus terrae (strain DSM 11246 / PB90-1)
UniProt IDB1ZVI7
Gene NamemiaB; Locus:Oter_0796
Protein Name
Region Expressed1-480
Expression Tag6xHis
Purity>90%
AA SequenceMNRVHIKTYGCQMNERDSEAVAAMLRARGYRIVADENDCDILLLNTCSVRDAAEQKAIGK AGYLQQRKKKQPDFVLGILGCMAQNRGASLLDQLPDVDLIVGTQKFHQVPGYLDNLRAAR DAGVPIGETIVDIGEEAGSQNTIKDHLLPQDSDSDSQPSTLNSQLRGAAAPPPQITAFVS IQQGCNMDCAFCIVPKTRGDERSRPMDDIVRECEQLAARGVREVTLLGQIVTSYGRRDYT HTNGISPFVQLLERVHALDGIERIRFTSPHPRGFKDDLVAAYGRLPKLCGYVHLPLQSGS NRILRAMNRPYTRERYREIVDALRAVRSDMYFSTDVIVGFPGETDEDFEQTRELFEACNY DMAYVFKYSVRTGTPAAERGDQVPEDVKEQRNQLLLELLRQNSERRNALLLDTVEEVLVE GPDKTGQRFTGRTRGNRVCIFEATPDLVGRLVSLRITRASVSTLYGELMLAGVGRERNRK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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