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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP476136BZOYeast1mgQuote
EP476136BZOE. coli1mgQuote
BP476136BZOBaculovirus200ugQuote
MP476136BZOMammalian Cell200ugQuote

Protein Information

SpeciesAnoxybacillus flavithermus (strain DSM 21510 / WK1)
UniProt IDB7GJM6
Gene NamemiaB; Locus:Aflv_1523
Protein Name
Region Expressed1-527
Expression Tag6xHis
Purity>90%
AA SequenceMMNEKQRLQYTAQIETDHPTDKKSALDENLQRLKQKTSQDYEKYFSTVFVPPSLKEAKKR GKEEVKYHKDFSIPEEFRGMGNGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPED ANVILLNTCAIRENAENKVFGEIGHLKPLKQNNPDLLLGVCGCMSQEESVVNKILKQFQY VDMIFGTHNIHRLPYILKEAYMSKEMVVEVWSKEGDVIENLPKVRKGNIKAWVNIMYGCD KFCTYCIVPYTRGKERSRRPEDIIQEVRHLAAQGYKEITLLGQNVNAYGKDFTDMKYGLG DLMDELRKIDIPRIRFTTSHPRDFDDRLIEVLAKGGNLVEHIHLPVQSGSSEVLKMMARK YTREQYLELVRKIKEAIPGVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIYSP REGTPAAKMVDNVPMEVKKERLQRLNALVNEISAKKMKEYEGQVVEVLVEGESKNNPDVL AGYTRKNKLVNFVGPKSAIGQLVNVRITEAKTWTLNGEMVEETIEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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