Request QuoteCatalog Number: xP470228EYXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP470228EYXYeast1mgQuote
EP470228EYXE. coli1mgQuote
BP470228EYXBaculovirus200ugQuote
MP470228EYXMammalian Cell200ugQuote

Protein Information

SpeciesProsthecochloris aestuarii (strain DSM 271 / SK 413)
UniProt IDB4S564
Gene NamemiaB; Locus:Paes_1999
Protein Name
Region Expressed1-441
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVSIRTFGCQMNQADTEIISSLLTAEGYCLVAEEDDADLVMLNTCAVRENAVEKIMH HLDSLKGLRRRRRGLVVGVIGCVPQYYREEMFQKSGVIDFIAGPDSYRKLPGLIRNAFQG IRGAELFLTQSETYGDIEPMRSGSISAFIPVMRGCNNMCAFCVVPFTRGRERSRSLQSVL DEVRRLEGQGYRELTLLGQNVNSYRDDEAGADFALLLDEVSKAAGEMRVRFTTSHPKDIS EDLVNVVAQRENVCNAIHLPVQSGSSRMLEVMHRGHTRREYLDKIAMIRSAVPGVALSTD LIAGFCGETEEDHLQTLSLMEEVRFDYAYMFYYSVRPGTYAARHLTDDVALEEKKRRLQE IIDLQSGISGEIFGNDVGSVQEVLAESESRRSSDMLMGRSDTNRVVVFDRQHYQPGDLVR VVIESSTQATLIGRCQDPGEG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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