Request QuoteCatalog Number: xP452055LOTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP452055LOTYeast1mgQuote
EP452055LOTE. coli1mgQuote
BP452055LOTBaculovirus200ugQuote
MP452055LOTMammalian Cell200ugQuote

Protein Information

SpeciesLeptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6) )
UniProt IDB1XYX5
Gene NamemiaB; Locus:Lcho_0584
Protein Name
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMHTPTLTSTPTTATSVVDDTAPAASGKKVYIRTFGCQMNEYDSDKMSDVMAAAEGYTPTD DPEQADLILFNTCSVREKAQEKVFSDLGRVKHLKARGVLIGVGGCVASQEGAAIIERAPY VDVVFGPQTLHRLPQMLAQRARQGRPQVDISFPEIEKFDHLPPAKVDGAAAFVSIMEGCS KYCSYCVVPYTRGEEFSRPFDEVLTEVAGLADQGVKEVTLLGQNVNAYRGRMGDTSDIAD FATLLEYVHEIPGIERIRYTTSHPNEFTPALIEAYARLPKLVNHLHLPVQHGSDRILSGM KRGYTVLEYKSTIRKLRAIRPDISLSTDFIVGFPGETDDDHARTMKLIDDIGFDASFSFI FSPRPGTPAAALHDDTSYEVKLARLQQLQATIEDNVRRISERRVGTVQRVLVEGPSRRDP NELMGRTECNRIVNFDGGPNAARLVGRMIDIRITLAYPHSLRGEPVLRD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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