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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP544509MVFYeast1mgQuote
EP544509MVFE. coli1mgQuote
BP544509MVFBaculovirus200ugQuote
MP544509MVFMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium abscessus (strain ATCC 19977 / DSM 44196)
UniProt IDB1MD05
Gene NamemiaB; Locus:MAB_3048c
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-507
Expression Tag6xHis
Purity>90%
AA SequenceMTSTATSGESAVRTYQVRTYGCQMNVHDSERVSGLLDAAGYVKAPEGTDADIVIFNTCAV RENADNKLYGNISHLAPRKAANPNMQIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSL PALLERARHNNEAQVEIVEALEHFPSALPATRESAYAAWVSISVGCNNTCTFCIVPSLRG KEIDRRPGDILGEVQALVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELLRACGR IDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPQLHMPLQSGSDRILKAMRRSYRSERFL SIIDKVRSAMPDAAITTDIIVGFPGETEEDFQQTLEVVRRARFSSAFTFQYSIRPGTPAA KLPDQLPKAVVQERYDRLIALQESVTLEENQKQIGRMIEVLIATGEGRKDGETARMSGRA RDGRLVHFRPQGHVDGALRPGDIITVDVTGAAPHHLIADDGVRSHRRTRAGDAHEAGKKP STPGIGLGMPAIGAPKTERIEVAGCGL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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