Request QuoteCatalog Number: xP543412MTDSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP543412MTDYeast1mgQuote
EP543412MTDE. coli1mgQuote
BP543412MTDBaculovirus200ugQuote
MP543412MTDMammalian Cell200ugQuote

Protein Information

SpeciesMethylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831)
UniProt IDB1LWE8
Gene NamemiaB; Locus:Mrad2831_0639
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMKKAFVKSYGCQMNAYDAARMADVLGAEGYTATDTVEDADVVVLNTCHIREKAAEKVYSE LGRLRVLKGERKAQGLDTRIVVAGCVAQAEGAEIQARQPAVDVVVGPQSYHRLPDLLARS RERRVVDTEFPVEDKFDHLPRRRTLGASAFLTVQEGCDKFCAFCVVPYTRGAEVSRSVAA VLAEAEKLADGGVREITLIGQNVNAYHGDGPDGAPAGLADLVRAVAKIPGIARIRYTTSH PNDFDDALIRAHAEVPALMPYLHLPVQSGSDRILAAMNRKHTGDAYRRLIDRIRAARPDI ALSSDFIVGFPGETDADFAETLRLVRDVGFESAFSFKYSTRPGTPAADRTDQVPEAVMAA RLAELQALLDSQRHAYQRAAAGRVFDVLVEKRGRHPGQVAGKTPHLLAVQFDAAPHHIGQ VVPVRITEAGSNSLFGELVSEAAAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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