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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP478593BQOYeast1mgQuote
EP478593BQOE. coli1mgQuote
BP478593BQOBaculovirus200ugQuote
MP478593BQOMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain B4264)
UniProt IDB7HDP7
Gene NamemiaB; Locus:BCB4264_A3871
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMNEQQRLASQQANSSTKKEEKDYSKYFENVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK VFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIP DVVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK KERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSL IGQLVKVKITEAKTWSLNGELVEEPIEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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