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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB 2

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB 2 can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP456255MVSYeast1mgQuote
EP456255MVSE. coli1mgQuote
BP456255MVSBaculovirus200ugQuote
MP456255MVSMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium marinum (strain ATCC BAA-535 / M)
UniProt IDB2HL08
Gene NamemiaB2; Locus:MMAR_1979
Protein Name
Region Expressed1-532
Expression Tag6xHis
Purity>90%
AA SequenceMTSTVAHGAGSAGPADDAEPMSARTYQVRTYGCQMNVHDSERMAGLLEAAGYRRAAEGTD ADVVVFNTCAVRENADNKLYGNLSHLAPRKRTSPDMQIAVGGCLAQKDRDALLRKAPWVD VVFGTHNIGSLPALLDRARHNRVAQVEIAEALQQFPSSLPSARESAYAAWVSISVGCNNT CTFCIVPSLRGKEIDRSPADILAEVQSLVDTGVVEITLLGQNVNAYGVSFADPALPRNRG AFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDRVLRA MRRSYRAERYLGIIERVRAAMPHAAITTDLIVGFPGETEQDFAATLDVVRQARFSAAFTF QYSKRPGTPAAELDGQLPKAVVQERYERLVELQEQISLEGNRAIVGQRVELLVATGEGRK DTLTARMSGRARDGRLVHFRAGDGPVRPGDIVTVEVTDAAPHHLIADGGILAHRRTRAGD AHADGQTVRGIGLGMPGIGRPVVPVAAEATSCGSAGGCGSADGAGSSAGDPQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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