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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP422300RMRYeast1mgQuote
EP422300RMRE. coli1mgQuote
BP422300RMRBaculovirus200ugQuote
MP422300RMRMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia canadensis (strain McKiel)
UniProt IDA8F011
Gene NamemiaB; Locus:A1E_05140
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLYIKTYGCQMNVYDSVKMQDLLYPFGYESTENIKEADVIILNTCHIREKAAEKIYS ELGRIKKLQDTRKKHGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELIS KVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGASAFISVQEGCDKFCTFCVVPYTRGVEFS RNVEQVYREALKVVSNGAREIMLLGQNVNAYHGKGVDDKIFSLADLIRYLAQIPNLERLR YTTSHPIDMTDDLIKLHSIESKLMPFVHLPVQSGSNKILKAMNRKHDREYYFNIINRLRE ARPDIVLSSDFIVGFPGETDEDFEDTLDLVRKIKYGQCYSFKYSPRPGTPGATRTDQIPE HIKSERLTILQKELSSQQLAFNESCVGSTMKVLFDRSGKFDGQIIGKTPYMQSVYINNTN QDLLCKIIDVKITKATSNSLTGEIYT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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