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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP420201AUYYeast1mgQuote
EP420201AUYE. coli1mgQuote
BP420201AUYBaculovirus200ugQuote
MP420201AUYMammalian Cell200ugQuote

Protein Information

SpeciesArcobacter butzleri (strain RM4018)
UniProt IDA8ERE9
Gene NamemiaB; Locus:Abu_0248
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMSSNKKLFIQTLGCQMNDTDSQHIQAELEKHKGYVTTQNIEDADLIIINTCSVRERPVQK LFSEIGQFNKKKKEGAKIGVCGCTASHLGQDIIKRAPYVDFVVGARNISKIKDVVDVKGA VEVSIDNDESTYEFSTAKTNKYRASVNISVGCDKKCTYCIVPSTRGEEISIPPEMIVEQV RKSVEQGAVEVMLLGQNVNSYGRKFSDKREKYTFTKLLQDVSKIDGLERIRFTSPHPLHM DDEFIEEFAKNPKISKCIHMPLQSGSTSVLKAMKRGYSKEWFLNRASKMRELVPNLRITT DIIVAFPGETQEDFLDTLDVVEQVKFDQIFNFKYSPRPGTEALNLKDKELPDEIGSQRLI DLIELHKRYLEESMPKLIGETLNILVESLKPNGEVCGYTDNYLQVFAKGSDELLGKFVNV KITDVTRTSLKGEVVN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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