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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP535863MTQYeast1mgQuote
EP535863MTQE. coli1mgQuote
BP535863MTQBaculovirus200ugQuote
MP535863MTQMammalian Cell200ugQuote

Protein Information

SpeciesMicrocystis aeruginosa (strain NIES-843)
UniProt IDB0JVM6
Gene NamemiaB; Locus:MAE_47970
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMNKSPRRYHITTFGCQMNKADSERMAGILEDLGFQWSEDANEADLILYNTCTIRDNAEQK VYSYLGRQAKRKQTQPDLTLIVAGCVAQQEGEQLLRRVPEVDLIIGPQHANRLGDLLQQV FDGSQVVATEPIHIMEDITKPRRDSNITAWVNVIYGCNERCTYCVVPGVRGVEQSRTPAA IRAEMAQLGQQGYQEITLLGQNIDAYGRDLPGVTASGRHLHNFTDLLYYVHDVAGIERLR FATSHPRYFTERLIKACQELPKVCEHFHIPFQSGDNDILKAMKRGYTQEKYRQIIANIRD LMPDAAISADAIVGFPGETEAQFENTLKLVDEIGFDQLNTAAYSPRPGTPAAIWDDQLSE QVKSDRLQRLNHLVATKAAERSQRYLGRIEEILVEDVNPKDASQVMGRTRGNRLTFFTGD IEELRGKFVKVKITEVRPFSLTGVIF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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