Request QuoteCatalog Number: xP535817BWUSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP535817BWUYeast1mgQuote
EP535817BWUE. coli1mgQuote
BP535817BWUBaculovirus200ugQuote
MP535817BWUMammalian Cell200ugQuote

Protein Information

SpeciesBrucella suis (strain ATCC 23445 / NCTC 10510)
UniProt IDB0CK00
Gene NamemiaB; Locus:BSUIS_A1991
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDA DLVLLNTCHIREKASEKLYSALGRLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAP NVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHLPAPRREETRKRGVSAFLTV QEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG REWGLGELLFRLARIPGIARLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRI LKAMNRRHKADEYLRLIERIRNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQA YSFKYSPRLGTPGADLDDHVEEAVKDERLQRLQALLSAQQYAFQDSMIGRKMDVLLEKPG REAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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