Request QuoteCatalog Number: xP535743RNBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP535743RNBYeast1mgQuote
EP535743RNBE. coli1mgQuote
BP535743RNBBaculovirus200ugQuote
MP535743RNBMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia rickettsii (strain Iowa)
UniProt IDB0BVC7
Gene NamemiaB; Locus:RrIowa_1456
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLYIKTYGCQMNVYDSIKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYS ELGRIKKLQDTRTKQGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELIS KVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGTSAFISVQEGCDKFCTFCVVPYTRGAEFS RNVEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLR YTTSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLRE ARPDIVLSSDFIVGFPGETDEDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPE HIKSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPN KSLLGKIIDVKITKASLNSLTGEIL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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