Request QuoteCatalog Number: xP432475DSXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP432475DSXYeast1mgQuote
EP432475DSXE. coli1mgQuote
BP432475DSXBaculovirus200ugQuote
MP432475DSXMammalian Cell200ugQuote

Protein Information

SpeciesChloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
UniProt IDA9WHB1
Gene NamemiaB; Locus:Caur_2414
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMQDVIFFDQRPRPDRDQTPRERRYYVWTVGCQMNISDSERLEAALQGVGYSPATRPEDAS FIVLNSCSVRASAEERILGKLGELVRVKRQHPDTRIVLWGCMVGPNNRSIFADQLPMVDH FVSPSAVDEVVALAPNPIYTLDEPALPVRDWSHPPVSVHVPIQYGCNMTCSYCVIPLRRG RERSRPLAEIVEEVRRIVARGAKEITLLGQIVDSWGHDLPGRPELADLLEAVDPTPGLLR LRFLTSHPAWMTDRLIETVARLPRCQPEINLPVQAGSDRVLKLMRRGYTVARYKTLIARI RAAIPDISLTTDIIVGHPGETEDDFRQTMDLCAEIGFDKVHIAAFSARPGTRAAEQEQDP ALAVPPAVKEERRRRLEQLQEQIATERMARFLGQTVEVLVEGESKGKWRGRTPGNRLVFF SHPADLTGQLVPVKITATSPWSLQGVPQLDDSVNAQSKENGLVKAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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