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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP435057MNYYeast1mgQuote
EP435057MNYE. coli1mgQuote
BP435057MNYBaculovirus200ugQuote
MP435057MNYMammalian Cell200ugQuote

Protein Information

SpeciesMethylobacterium extorquens (strain PA1)
UniProt IDA9VYZ9
Gene NamemiaB; Locus:Mext_3204
Protein Name
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMKKAYVKSYGCQMNAYDAGRMADVLAAEGYSATDTVEEADVVVLNTCHIREKAAEKVYSE LGRLRVLKGERAESGHDTRIVVAGCVAQAEGREILSRAPAVDVVVGPQSYHRLPDLLRQS RETRVVDTEFPAEDKFDHLPARRNRGVTGFLTVQEGCDKFCAFCVVPYTRGAEVSRSVAA VVDEARRLVEGGVREITLIGQNVNAYHGNGPDGAPATLGHLMDALSAVPGLLRLRYTTSH PNDFADDLIAAHATNPLVMPYLHLPVQSGSDRILHAMNRRHTGDAYRRLIERIRNARPDI ALSSDFIVGFPGETDADFAETMRLVSDIGFSAAFSFKYSPRAGTPAAEREDAVPEAVKTE RLAALQDLLDRQRHAYNAASVGTLTEILVEKTGRHPGQVAGKTPHLQAVQFDAPASTIGT VVPVRITRAGSNSLFGETLEGAAAAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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